Phospho-GAB1-Y659 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-GAB1-Y659 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0774
• Supplier Catalog Number: AP0774
• Alternative Catalog Number: ABB-AP0774-20UL, ABB-AP0774-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: DFNB26, Phospho-GAB1-Y659

Product Description

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 77kDa
• NCBI: 2549
• UniProt: Q13480
• Purity: Affinity purification
• Target: GAB1

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,Cell differentiation,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-GAB1-Y659 Rabbit pAb (AP0774) at 1:2000 dilution. NIH/3T3 cells were treated with Insulin (100nM) for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.