Phospho-PFKFB2-S483 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PFKFB2-S483 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0784
• Supplier Catalog Number: AP0784
• Alternative Catalog Number: ABB-AP0784-20UL,ABB-AP0784-100UL,ABB-AP0784-1000UL,ABB-AP0784-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PFK-2/FBPase-2, Phospho-PFKFB2-S483

Product Description

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 58kDa
• NCBI: 5208
• UniProt: O60825
• Purity: Affinity purification
• Sequence: NYSVG
• Target: PFKFB2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,PI3K-Akt Signaling Pathway,Endocrine Metabolism,Carbohydrate metabolism,Warburg Effect.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-PFKFB2-S483 Rabbit pAb (AP0784) at 1:2000 dilution. HeLa cells were treated with IGF-1 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.