Phospho-SHP1-Y564 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-SHP1-Y564 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0787
• Supplier Catalog Number: AP0787
• Alternative Catalog Number: ABB-AP0787-20UL, ABB-AP0787-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HCP, HCPH, SHP1, SHP-1, HPTP1C, PTP-1C, SHP-1L, SH-PTP1, Phospho-SHP1-Y564

Product Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.

Product Properties

• Molecular Weight: 68kDa
• NCBI: 5777
• UniProt: P29350
• Purity: Affinity purification
• Target: PTPN6

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled ReceptorsGPCR,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,Immunology Inflammation,B Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway.

Images

Western blot analysis of lysates from HeLa and RAW 264.7 cells, using Phospho-SHP1-Y564 Rabbit pAb (AP0787) at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37C for 1 hour.RAW 264.7 cell lysates were treated by CIP at 37C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.