Phospho-CBL-Y774 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-CBL-Y774 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0794
• Supplier Catalog Number: AP0794
• Alternative Catalog Number: ABB-AP0794-20UL, ABB-AP0794-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CBL2, NSLL, C-CBL, RNF55, FRA11B, Phospho-CBL-Y774

Product Description

This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5 UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder.

Product Properties

• Molecular Weight: 100kDa
• NCBI: 867
• UniProt: P22681
• Purity: Affinity purification
• Target: CBL

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Nuclear Receptor Signaling,Nuclear hormone receptors,Protein phosphorylation,Cancer,Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-CBL-Y774 Rabbit pAb (A0732). Jurkat cells were treated with Pervanadate (1 mM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30S.