Phospho-Vimentin-S83 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-Vimentin-S83 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0799
• Supplier Catalog Number: AP0799
• Alternative Catalog Number: ABB-AP0799-20UL, ABB-AP0799-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CTRCT30, HEL113, Vimentin, VIM, vimentin, Phospho-Vimentin-S83

Product Description

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Product Properties

• Molecular Weight: 54kDa
• NCBI: 7431
• UniProt: P08670
• Purity: Affinity purification
• Target: VIM

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Rat, ResearchArea: Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Intermediate Filaments,Neuroscience, Cell Type Marker,Stem Cells,Neural Stem Cells.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 pAb (AP0799) at 1:2000 dilution or Vimentin antibody (A2584). HeLa cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours or treated with Paclitaxel (100 nM/mL) at 37°C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.