Phospho-CDK9-T186 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-CDK9-T186 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0810
• Supplier Catalog Number: AP0810
• Alternative Catalog Number: ABB-AP0810-20UL,ABB-AP0810-100UL,ABB-AP0810-500UL,ABB-AP0810-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: TAK, C-2k, CTK1, CDC2L4, PITALRE, Phospho-CDK9-T186

Product Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 43kDa
• NCBI: 1025
• UniProt: P50750
• Purity: Affinity purification
• Sequence: RYTNR
• Target: CDK9
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Cell Cycle,Immunology Inflammation.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK9-T186 Rabbit pAb (AP0810) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.