Phospho-PDGFRB-Y1021 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PDGFRB-Y1021 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0815
• Supplier Catalog Number: AP0815
• Alternative Catalog Number: ABB-AP0815-20UL, ABB-AP0815-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: IMF1, KOGS, IBGC4, JTK12, PDGFR, PENTT, CD140B, PDGFR1, PDGFR-1, Phospho-PDGFRB-Y1021

Product Description

The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor, all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.

Product Properties

• Molecular Weight: 124kDa
• NCBI: 5159
• UniProt: P09619
• Purity: Affinity purification
• Target: PDGFRB

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Growth factors,Immunology Inflammation,CDs,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PDGFRB-Y1021 Rabbit pAb (AP0815) at 1:1000 dilution. NIH/3T3 cells were treated with PDGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.