Phospho-LCP2-Y145 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-LCP2-Y145 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0816
• Supplier Catalog Number: AP0816
• Alternative Catalog Number: ABB-AP0816-100UL, ABB-AP0816-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: IMD81, SLP76, SLP-76, Phospho-LCP2-Y145

Product Description

This gene encodes an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. The encoded protein associates with growth factor receptor bound protein 2, and is thought to play a role TCR-mediated intracellular signal transduction. A similar protein in mouse plays a role in normal T-cell development and activation. Mice lacking this gene show subcutaneous and intraperitoneal fetal hemorrhaging, dysfunctional platelets and impaired viability.

Product Properties

• Molecular Weight: 60kDa
• NCBI: 3937
• UniProt: Q13094
• Purity: Affinity purification
• Target: LCP2

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Signal Transduction,Immunology Inflammation,T Cell Receptor Signaling Pathway.

Images

Western blot analysis of lysates from U937 cells, using Phospho-LCP2-Y145 Rabbit pAb (AP0816) at 1:1000 dilution. U-937 cells were treated with PMA/TPA (200nM) for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.