Phospho-CRK/CRKII-Y221 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-CRK/CRKII-Y221 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0822
• Supplier Catalog Number: AP0822
• Alternative Catalog Number: ABB-AP0822-20UL, ABB-AP0822-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: p38, CRKII, Phospho-CRK/CRKII-Y221

Product Description

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Product Properties

• Molecular Weight: 34kDa
• NCBI: 1398
• UniProt: P46108
• Purity: Affinity purification
• Target: CRK

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation.

Images

Western blot analysis of lysates from K-562 cells, using Phospho-CRK/CRKII-Y221 Rabbit pAb (AP0822) at 1:1000 dilution. K-562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.