Phospho-AP2M1-T156 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-AP2M1-T156 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0823
• Supplier Catalog Number: AP0823
• Alternative Catalog Number: ABB-AP0823-20UL, ABB-AP0823-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: mu2, AP50, MRD60, CLAPM1, Phospho-AP2M1-T156

Product Description

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 50kDa
• NCBI: 1173
• UniProt: Q96CW1
• Purity: Affinity purification
• Target: AP2M1

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Signal Transduction,Neuroscience,Neurodegenerative Diseases.

Images

Western blot analysis of lysates from 293T and NIH/3T3 cells, using Phospho-AP2M1-T156 Rabbit pAb (AP0823) at 1:1000 dilution. 293T cells were treated with Calyculin A for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated with Hydrogen Peroxide (2nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.