Phospho-PLC beta 3 (PLCB3)-S1105 Rabbit pAb, Unconjugated

Catalog Number: ABB-AP0837
Article Name: Phospho-PLC beta 3 (PLCB3)-S1105 Rabbit pAb, Unconjugated
Biozol Catalog Number: ABB-AP0837
Supplier Catalog Number: AP0837
Alternative Catalog Number: ABB-AP0837-20UL, ABB-AP0837-100UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: SMDCD, Phospho-PLC beta 3 (PLCB3)-S1105
This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.
Molecular Weight: 139kDa
NCBI: 5331
UniProt: Q01970
Purity: Affinity purification
Target: PLCB3
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Endocrine Metabolism,Lipid Metabolism,AMPK Signaling Pathway,Neuroscience,Neurodegenerative Diseases.
Western blot analysis of lysates from HeLa cells, using Phospho-PLC beta 3 (PLCB3)-S1105 Rabbit pAb (AP0837) at 1:1000 dilution. HeLa cells were treated with PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.