Phospho-HSL-S660 Rabbit pAb, Unconjugated
Catalog Number:
ABB-AP0853
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| Article Name: | Phospho-HSL-S660 Rabbit pAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP0853 |
| Supplier Catalog Number: | AP0853 |
| Alternative Catalog Number: | ABB-AP0853-20UL,ABB-AP0853-100UL,ABB-AP0853-1000UL,ABB-AP0853-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | HSL, LHS, REH, AOMS4, FPLD6, Phospho-HSL-S660 |
| The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Endocrine Metabolism,Lipid Metabolism,Cholesterol Metabolism,Lipases,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Cardiovascular,Lipids. |
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Western blot analysis of lysates from 3T3-L1 cells, using Phospho-HSL-S660 Rabbit pAb (AP0853) at 1:600 dilution. 3T3-L1 cells were treated with Isoproterenol (10 µM for 20 min) at 37°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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