Phospho-PEA15-S104 Rabbit pAb, Unconjugated

Catalog Number: ABB-AP0874
Article Name: Phospho-PEA15-S104 Rabbit pAb, Unconjugated
Biozol Catalog Number: ABB-AP0874
Supplier Catalog Number: AP0874
Alternative Catalog Number: ABB-AP0874-100UL,ABB-AP0874-20UL,ABB-AP0874-1000UL,ABB-AP0874-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC-P, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: PED, MAT1, HMAT1, MAT1H, PEA-15, HUMMAT1H, PED-PEA15, PED/PEA15, Phospho-PEA15-S104
This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.
Clonality: Polyclonal
Molecular Weight: 15kDa
NCBI: 8682
UniProt: Q15121
Purity: Affinity purification
Sequence: IPSAK
Target: PEA15
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:2000|IHC-P,1:50 - 1:100|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis.
Western blot analysis of various lysates using Phospho-PEA15-S104 Rabbit pAb (AP0874) at 1:1000 dilution. C6 cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.