Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb

Product Overview

• Article Name: Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb
• Biozol Catalog Number: ABB-AP0933
• Supplier Catalog Number: AP0933
• Alternative Catalog Number: ABB-AP0933-100UL,ABB-AP0933-20UL,ABB-AP0933-500UL,ABB-AP0933-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.

Product Description

The functionally related ATM (ataxia telangiectasia-mutated) and ATR (ATM-Rad3-related) protein kinases are critical regulators of DNA damage responses in mammalian cells. ATM and ATR share highly overlapping substrate specificities and show a strong preference for the phosphorylation of Serine (S) or Threonine (T) residues followed by Gln. It also called SQ or TQ consensus sites.

Product Properties

• Clonality: Polyclonal
• Purity: Affinity purification
• Target: pan p-S/T
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Protein phosphorylation,Protein phosphorylation.

Images

Western blot analysis of various lysates using Phospho-(Ser/Thr) ATM/ATR Substrate pAb (AP0933) at 1:1000 dilution. Jurkat and NIH/3T3 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. C6 cells were treated with ATP(5 mM) at 30°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.