Phospho-SMO-S611/615 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-SMO-S611/615 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0939
• Supplier Catalog Number: AP0939
• Alternative Catalog Number: ABB-AP0939-100UL, ABB-AP0939-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: Gx, CRJS, PHLS, SMOH, FZD11, Phospho-SMO-S611/615

Product Description

The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.

Product Properties

• Molecular Weight: 86kDa
• NCBI: 6608
• UniProt: Q99835
• Purity: Affinity purification
• Target: SMO

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Signal Transduction,G protein signaling,G-Protein-Coupled ReceptorsGPCR,Cell Biology Developmental Biology,Apoptosis,Neuroscience,Stem Cells.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-SMO-S611/615 Rabbit pAb (AP0939) at 1:1000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes. Jurkat cells were treated with Anisomycin (25 µg/mL) at 37°C for 20 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.