Phospho-AURKA-T288/AURKB-T232/AURKC-T198 Rabbit pAb
Catalog Number:
ABB-AP0948
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| Article Name: | Phospho-AURKA-T288/AURKB-T232/AURKC-T198 Rabbit pAb |
| Biozol Catalog Number: | ABB-AP0948 |
| Supplier Catalog Number: | AP0948 |
| Alternative Catalog Number: | ABB-AP0948-20UL,ABB-AP0948-100UL,ABB-AP0948-500UL,ABB-AP0948-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Alternative Names: | AURKA/AURKB/AURKC, Phospho-AURKA-T288/AURKB-T232/AURKC-T198 |
| The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Application Dilute: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Rat, ResearchArea: Protein phosphorylation. |
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Western blot analysis of lysates from HeLa cells, using Phospho-AURKA-T288/AURKB-T232/AURKC-T198 Rabbit pAb (AP0948) at 1:1000 dilution. Hela cells were treated with ATP(5 mM) at 30°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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