Phospho-GAB1-Y307 Rabbit pAb, Unconjugated
Catalog Number:
ABB-AP0961
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| Article Name: | Phospho-GAB1-Y307 Rabbit pAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP0961 |
| Supplier Catalog Number: | AP0961 |
| Alternative Catalog Number: | ABB-AP0961-20UL,ABB-AP0961-100UL,ABB-AP0961-500UL,ABB-AP0961-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | DFNB26, Phospho-GAB1-Y307 |
| The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Mouse, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,Cell differentiation,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway. |
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Western blot analysis of lysates from NIH/3T3 cells usingPhospho-GAB1-Y307 Rabbit pAb (AP0961) at1:1000 dilution. NIH/3T3 cells were treated with IGF-1 (50 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:60s. |
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