Phospho-PKC alpha-S657/Y658 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PKC alpha-S657/Y658 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0970
• Supplier Catalog Number: AP0970
• Alternative Catalog Number: ABB-AP0970-100UL,ABB-AP0970-20UL,ABB-AP0970-500UL,ABB-AP0970-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: AAG6, PKCA, PRKACA, PKCI+/-, PKCalpha, PKC-alpha, Phospho-PKC alpha-S657/Y658

Product Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 77kDa
• NCBI: 5578
• UniProt: P17252
• Purity: Affinity purification
• Sequence: GFSYVN
• Target: PRKCA
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Serine threonine kinases,mTOR Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Cell Adhesion,Cytoskeleton,Microtubules,Actins,TGF-b-Smad Signaling Pathway,Endocrine Metabolism,Immunology Inflammation,B Cell Receptor Signaling Pathway,Neuroscience,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Neurodegenerative Diseases Markers,Cardiovascular,Heart,Hypertrophy.

Images

Western blot analysis of various lysates using Phospho-PKC alpha-S657/Y658 Rabbit pAb (AP0970) at 1:1000 dilution. 293T cells were treated with CIP(20uL/400ul) at 37°C for 1 hour. 293T cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.