Phospho-Smad5-S463/S465 Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1023
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| Article Name: | Phospho-Smad5-S463/S465 Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1023 |
| Supplier Catalog Number: | AP1023 |
| Alternative Catalog Number: | ABB-AP1023-100UL,ABB-AP1023-20UL,ABB-AP1023-1000UL,ABB-AP1023-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | DWFC, JV5-1, MADH5, Phospho-Smad5-S463/S465 |
| The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Signal Transduction,Cell Biology Developmental Biology,TGF-b-Smad Signaling Pathway,ESC Pluripotency and Differentiation,Stem Cells. |
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Western blot analysis of lysates from HeLa cells, using Phospho-Smad5-S463/S465 Rabbit mAb (AP1023) at 1:1000 dilution or Smad5 antibody (A1947). Hela cells were treated with ATP(5 mM) at 30°C for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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