Phospho-TBK1/NAK-S172 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-TBK1/NAK-S172 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1026
• Supplier Catalog Number: AP1026
• Alternative Catalog Number: ABB-AP1026-20UL,ABB-AP1026-100UL,ABB-AP1026-500UL,ABB-AP1026-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: NAK, T2K, IIAE8, FTDALS4, Phospho-TBK1/NAK-S172

Product Description

The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1571]
• Molecular Weight: 84kDa
• NCBI: 29110
• UniProt: Q9UHD2
• Purity: Affinity purification
• Sequence: FVSLY
• Target: TBK1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,Immunology Inflammation,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway.

Images

Western blot analysis of various lysates using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1026) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.