Phospho-HSP27/HSPB1-S78 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-HSP27/HSPB1-S78 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1032
• Supplier Catalog Number: AP1032
• Alternative Catalog Number: ABB-AP1032-20UL,ABB-AP1032-100UL,ABB-AP1032-1000UL,ABB-AP1032-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, Phospho-HSP27/HSPB1-S78

Product Description

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1577]
• Molecular Weight: 23kDa
• NCBI: 3315
• UniProt: P04792
• Purity: Affinity purification
• Sequence: ALSRQ
• Target: HSPB1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cytoskeleton,Actins,Stem Cells,Cardiovascular,Heart,Contractility.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit mAb (AP1032) at 1:1000 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.