Phospho-RPA2-T21 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-RPA2-T21 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1040
• Supplier Catalog Number: AP1040
• Alternative Catalog Number: ABB-AP1040-100UL,ABB-AP1040-20UL,ABB-AP1040-1000UL,ABB-AP1040-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: REPA2, RPA32, RP-A p32, RP-A p34, Phospho-RPA2-T21

Product Description

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1586]
• Molecular Weight: 29kDa
• NCBI: 6118
• UniProt: P15927
• Purity: Affinity purification
• Sequence: GYTQS
• Target: RPA2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Rat, ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair.

Images

Western blot analysis of lysates from C6 cells, using Phospho-RPA2-T21 Rabbit mAb (AP1040) at 1:1000 dilution. C6 cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.