Phospho-PP2A Catalytic alpha-Y307 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-PP2A Catalytic alpha-Y307 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1043
• Supplier Catalog Number: AP1043
• Alternative Catalog Number: ABB-AP1043-20UL,ABB-AP1043-100UL,ABB-AP1043-1000UL,ABB-AP1043-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: RP-C, PP2Ac, PP2CA, NEDLBA, PP2Calpha, Phospho-PP2A Catalytic alpha-Y307

Product Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1589]
• Molecular Weight: 36kDa
• NCBI: 5515
• UniProt: P67775
• Purity: Affinity purification
• Sequence: PDYFL
• Target: PPP2CA
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse, ResearchArea: Cancer,Signal Transduction,G protein signaling,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,TGF-b-Smad Signaling Pathway,Wnt -Catenin Signaling Pathway,ESC Pluripotency and Differentiation,Endocrine Metabolism,Carbohydrate metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PP2A Catalytic alpha-Y307 Rabbit mAb (AP1043) at 1:1000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.