Phospho-Chk1-S296 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-Chk1-S296 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1047
• Supplier Catalog Number: AP1047
• Alternative Catalog Number: ABB-AP1047-100UL,ABB-AP1047-20UL,ABB-AP1047-500UL,ABB-AP1047-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CHK1, Phospho-Chk1-S296

Product Description

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1593]
• Molecular Weight: 54kDa
• NCBI: 1111
• UniProt: O14757
• Purity: Affinity purification
• Sequence: IQSNL
• Target: CHEK1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,Protein phosphorylation,Cancer,Signal Transduction,Kinase,Serine threonine kinases,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,Cell Cycle Control-G1 S Checkpoint,Cell Cycle Control-G2 M DNA Damage Checkpoint.

Images

Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S296 Rabbit mAb (AP1047) at 1:1000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes and Doxorubicin (0. 5 uM) at 37°C for 24 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.