Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1049
• Supplier Catalog Number: AP1049
• Alternative Catalog Number: ABB-AP1049-100UL,ABB-AP1049-20UL,ABB-AP1049-1000UL,ABB-AP1049-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PAK4, KIAA1142, PAK5KIAA1264, PAK6, Phospho-PAK4/PAK5/PAK6-S474/S560/S602

Product Description

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1596]
• Molecular Weight: 64, 80, 75 kDa
• NCBI: 10298
• UniProt: O96013
• Purity: Affinity purification
• Sequence: RKSLV
• Target: PAK4/PAK5/PAK6
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse,Rat, ResearchArea: Cell Biology,Apoptosis,Intracellular,Kinases,Signal Transduction, Cytoskeleton / ECM,Neuroscience.

Images

Western blot analysis of various lysates using Phospho-PAK4/PAK5/PAK6-S474/S560/S602 Rabbit mAb (AP1049) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.