Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
JTK8, p53Lyn, p56Lyn, Phospho-Lyn-Y396
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Mouse, ResearchArea: Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,PI3K-Akt Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Lyn-Y397 Rabbit mAb (AP1050) at 1:1000 dilution. NIH/3T3 cells were treated with pervanadate (1 mM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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