Phospho-ATF2-T71 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-ATF2-T71 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1051
• Supplier Catalog Number: AP1051
• Alternative Catalog Number: ABB-AP1051-100UL,ABB-AP1051-20UL,ABB-AP1051-500UL,ABB-AP1051-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HB16, CREB2, TREB7, CREB-2, CRE-BP1, Phospho-ATF2-T71

Product Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cells DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1598]
• Molecular Weight: 55kDa
• NCBI: 1386
• UniProt: P15336
• Purity: Affinity purification
• Sequence: TPTPT
• Target: ATF2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Signal Transduction,MAPK-JNK Signaling Pathway,ATM Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T71 Rabbit mAb (AP1051) at 1:1000 dilution. NIH/3T3 cells were treated with Anisomycin (25 µg/mL) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.