Phospho-ErbB3/HER3-Y1222 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-ErbB3/HER3-Y1222 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1052
• Supplier Catalog Number: AP1052
• Alternative Catalog Number: ABB-AP1052-100UL,ABB-AP1052-20UL,ABB-AP1052-500UL,ABB-AP1052-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HER3, FERLK, LCCS2, VSCN1, ErbB-3, c-erbB3, erbB3-S, MDA-BF-1, c-erbB-3, p180-ErbB3, p45-sErbB3, p85-sErbB3, Phospho-ErbB3/HER3-Y1222

Product Description

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1599]
• Molecular Weight: 148kDa
• NCBI: 2065
• UniProt: P21860
• Purity: Affinity purification
• Sequence: LGYEY
• Target: ERBB3
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,G protein signaling,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Growth factors,Immunology Inflammation.

Images

Western blot analysis of lysates from 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (AP1052) at 1:1000 dilution. 293T cells were treated with CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.