Phospho-FADD-S194 Rabbit pAb

Product Overview

• Article Name: Phospho-FADD-S194 Rabbit pAb
• Biozol Catalog Number: ABB-AP1054
• Supplier Catalog Number: AP1054
• Alternative Catalog Number: ABB-AP1054-100UL,ABB-AP1054-20UL,ABB-AP1054-1000UL,ABB-AP1054-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: GIG3, IMD90, MORT1, Phospho-FADD-S194

Product Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 23kDa
• NCBI: 8772
• UniProt: Q13158
• Purity: Affinity purification
• Sequence: AMSPM
• Target: FADD
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Invasion and Metastasis,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology Inflammation,Toll-like Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-FADD-S194 Rabbit pAb (A5819). Hela cells were treated with CIP(20uL/400ul) at 37°C for 1 hour.HeLa cells were treated with nocodazole (50 ng/mL) at 37°C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.