Phospho-C/EBPB-T235 Rabbit pAb

Product Overview

• Article Name: Phospho-C/EBPB-T235 Rabbit pAb
• Biozol Catalog Number: ABB-AP1055
• Supplier Catalog Number: AP1055
• Alternative Catalog Number: ABB-AP1055-20UL,ABB-AP1055-100UL,ABB-AP1055-1000UL,ABB-AP1055-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: TCF5, IL6DBP, NF-IL6, C/EBP-beta, Phospho-C/EBPB-T235

Product Description

This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 36kDa
• NCBI: 1051
• UniProt: P17676
• Purity: Affinity purification
• Sequence: PGTPS
• Target: CEBPB
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Signal Transduction,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Adhesion,Wnt -Catenin Signaling Pathway,Endocrine Metabolism,Endocrine and metabolic diseases,Diabetes,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway,Cardiovascular,Heart,Hypertrophy.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-C/EBPB-T235 Rabbit pAb (AP1055) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. NIH/3T3 cells were treated by Serum-starvation overnight at 37°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.