Phospho-IkappaBalpha-S36 Rabbit pAb

Product Overview

• Article Name: Phospho-IkappaBalpha-S36 Rabbit pAb
• Biozol Catalog Number: ABB-AP1069
• Supplier Catalog Number: AP1069
• Alternative Catalog Number: ABB-AP1069-100UL,ABB-AP1069-20UL,ABB-AP1069-1000UL,ABB-AP1069-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: IKBA, MAD-3, NFKBI, EDAID2, Phospho-IkappaBalpha-S36

Product Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 36kDa
• NCBI: 4792
• UniProt: P25963
• Purity: Affinity purification
• Sequence: LDSMK
• Target: NFKBIA
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,MAPK-Erk Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-IkappaBalpha-S36 Rabbit pAb (A11397). HeLa cells were treated with TNF-alpha (20 ng/mL) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.