Phospho-STK3/STK4-T180/T183 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-STK3/STK4-T180/T183 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1094
• Supplier Catalog Number: AP1094
• Alternative Catalog Number: ABB-AP1094-20UL,ABB-AP1094-500UL,ABB-AP1094-100UL,ABB-AP1094-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: KRS1, MST2, KRS2, MST1, YSK3

Product Description

This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Product Properties

• Molecular Weight: 56kDa
• NCBI: 6788
• UniProt: Q13188
• Purity: Affinity purification
• Sequence: RNTVI
• Target: STK3/STK4

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat

Images

Western blot analysis of various lysates, using Phospho-STK3/STK4-T180/T183 Rabbit pAb (AP1094) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated with Staurosporine(1uM) at room temperature for 3 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.