Phospho-VEGF Receptor 2-Y1175 Rabbit pAb

Product Overview

• Article Name: Phospho-VEGF Receptor 2-Y1175 Rabbit pAb
• Biozol Catalog Number: ABB-AP1095
• Supplier Catalog Number: AP1095
• Alternative Catalog Number: ABB-AP1095-20UL,ABB-AP1095-100UL,ABB-AP1095-1000UL,ABB-AP1095-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: FLK1, CD309, VEGFR, VEGFR2, Phospho-VEGF Receptor 2-Y1175

Product Description

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 152kDa
• NCBI: 3791
• UniProt: P35968
• Purity: Affinity purification
• Sequence: KDYIV
• Target: KDR
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation,Cancer,Invasion and Metastasis,Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Growth factors,Endocrine Metabolism,Immunology Inflammation,CDs,Stem Cells,Hematopoietic Progenitors,Cardiovascular,Angiogenesis,Hypoxia.

Images

Western blot analysis of various lysates using Phospho-VEGF Receptor 2-Y1175 pAb (AP1095) at 1:1000 dilution. HUVEC cells were treated with VEGF (100 ng/mL) at 37°C for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.