Phospho-p70 S6 Kinase 1-T421/S424 Rabbit pAb

Product Overview

• Article Name: Phospho-p70 S6 Kinase 1-T421/S424 Rabbit pAb
• Biozol Catalog Number: ABB-AP1106
• Supplier Catalog Number: AP1106
• Alternative Catalog Number: ABB-AP1106-100UL,ABB-AP1106-20UL,ABB-AP1106-1000UL,ABB-AP1106-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: S6K, PS6K, S6K1, STK14A, p70-S6K, p70 S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, Phospho-p70 S6 Kinase 1-T421/S424

Product Description

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 59kDa
• NCBI: 6198
• UniProt: P23443
• Purity: Affinity purification
• Sequence: RTPVSP
• Target: RPS6KB1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,DNA Damage Repair,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,Cell Cycle,Cell cycle inhibitors,TGF-b-Smad Signaling Pathway,Endocrine Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Obesity,Immunology Inflammation,B Cell Receptor Signaling Pathway,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T421/S424 pAb (AP1106) at 1:1000 dilution or P70 S6K antibody (A16968). NIH/3T3 cells were treated with EGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.