Phospho-STK11-S428 Rabbit pAb

Product Overview

• Article Name: Phospho-STK11-S428 Rabbit pAb
• Biozol Catalog Number: ABB-AP1108
• Supplier Catalog Number: AP1108
• Alternative Catalog Number: ABB-AP1108-100UL, ABB-AP1108-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: PJS, LKB1, hLKB1, Phospho-STK11-S428

Product Description

The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers.

Product Properties

• Molecular Weight: 49kDa
• NCBI: 6794
• UniProt: Q15831
• Purity: Affinity purification
• Target: STK11

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,Kinase,Serine threonine kinases,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Autophagy,Cell Cycle,Cell cycle inhibitors,Endocrine Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Warburg Effect.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STK11-S428 Rabbit pAb (AP1108) at 1:1000 dilution or LKB1 antibody (A2122). NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.