Phospho-TSC2-S1387 Rabbit pAb

Product Overview

• Article Name: Phospho-TSC2-S1387 Rabbit pAb
• Biozol Catalog Number: ABB-AP1117
• Supplier Catalog Number: AP1117
• Alternative Catalog Number: ABB-AP1117-20UL, ABB-AP1117-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: LAM, TSC4, PPP1R160, Phospho-TSC2-S1387

Product Description

This gene is a tumor suppressor gene that encodes the growth inhibitory protein tuberin. Tuberin interacts with hamartin to form the TSC protein complex which functions in the control of cell growth. This TSC protein complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signaling which is a major regulator of anabolic cell growth. Mutations in this gene have been associated with tuberous sclerosis and lymphangioleiomyomatosis.

Product Properties

• Molecular Weight: 201kDa
• NCBI: 7249
• UniProt: P49815
• Purity: Affinity purification
• Target: TSC2

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Cancer,Tumor suppressors,Signal Transduction,G protein signaling,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Autophagy,Cell Cycle,Cell cycle inhibitors,Endocrine Metabolism,AMPK Signaling Pathway,Insulin Receptor Signaling Pathway,Endocrine and metabolic diseases,Obesity,Immunology Inflammation.

Images

Western blot analysis of lysates from C2C12 cells, using Phospho-TSC2-S1387 Rabbit pAb (AP1117) at 1:1000 dilution. C2C12 cells were treated with CIP(20uL/400ul) at 37°C for 1 hour. C2C12 cells were treated with AICAR (0. 5 mM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.