Phospho-EZR-T567/RDX-T564/MSN-T558 Rabbit pAb

Catalog Number: ABB-AP1121
Article Name: Phospho-EZR-T567/RDX-T564/MSN-T558 Rabbit pAb
Biozol Catalog Number: ABB-AP1121
Supplier Catalog Number: AP1121
Alternative Catalog Number: ABB-AP1121-100UL,ABB-AP1121-20UL,ABB-AP1121-500UL,ABB-AP1121-1000UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Clonality: Polyclonal
Molecular Weight: 23-71 kDa
NCBI: 7430
UniProt: P15311
Purity: Affinity purification
Sequence: YKTLR
Target: EZR/RDX/MSN
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Rat, ResearchArea: Protein phosphorylation.
Western blot analysis of lysates from PC-12 cells, using Phospho-EZR-T567/RDX-T564/MSN-T558 Rabbit pAb (AP1121) at 1:1000 dilution. PC-12 cells were treated with ATP(5 mM) at 30°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.