Phospho-PKC zeta-T560 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-PKC zeta-T560 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1149
• Supplier Catalog Number: AP1149
• Alternative Catalog Number: ABB-AP1149-100UL,ABB-AP1149-20UL,ABB-AP1149-500UL,ABB-AP1149-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PKC2, PKC-ZETA, Phospho-PKC zeta-T560

Product Description

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1617]
• Molecular Weight: 68 kDa
• NCBI: 5590
• UniProt: Q05513
• Purity: Affinity purification
• Sequence: QLTPD
• Target: PRKCZ
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:3000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Rat, ResearchArea: Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Serine threonine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Mitochondrial Control of Apoptosis,Inhibition of Apoptosis,TGF-b-Smad Signaling Pathway,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,NF-kB Signaling Pathway.

Images

Western blot analysis of various lysates using Phospho-PKC zeta-T560 Rabbit mAb (AP1149) at 1:1000 dilution. Both 293T cells and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.