Phospho-Cdc6-S54 Rabbit mAb, Unconjugated

Product Overview

• Article Name: Phospho-Cdc6-S54 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-AP1153
• Supplier Catalog Number: AP1153
• Alternative Catalog Number: ABB-AP1153-100UL,ABB-AP1153-20UL,ABB-AP1153-500UL,ABB-AP1153-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: CDC18L, HsCDC6, MGORS5, HsCDC18, Phospho-Cdc6-S54

Product Description

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC1693]
• Molecular Weight: 63kDa
• NCBI: 990
• UniProt: Q99741
• Purity: Affinity purification
• Sequence: PLSPR
• Target: CDC6
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:1000 - 1:6000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Mouse, ResearchArea: Epigenetics Nuclear Signaling,Cell Biology Developmental Biology,Cell Cycle.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb (AP1153) at 1:1000 dilution. NIH/3T3 cells were treated with Paclitaxel (1 uM) at 37°C for 20 hours. NIH/3T3 cells were treated with Hydroxyurea (4 mM) at 37°C for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.