Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1158
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| Article Name: | Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1158 |
| Supplier Catalog Number: | AP1158 |
| Alternative Catalog Number: | ABB-AP1158-100UL,ABB-AP1158-20UL,ABB-AP1158-1000UL,ABB-AP1158-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | PAKalpha, Phospho-PAK1/2/3-S144/S141/S139 |
| This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat |
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Western blot analysis of various lysates using Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb (AP1158) at 1:1000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37°C for 30 minutes after serum-starvation overnight Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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