Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Rabbit pAb

Product Overview

• Article Name: Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Rabbit pAb
• Biozol Catalog Number: ABB-AP1164
• Supplier Catalog Number: AP1164
• Alternative Catalog Number: ABB-AP1164-20UL,ABB-AP1164-100UL,ABB-AP1164-1000UL,ABB-AP1164-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.

Product Description

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Product Properties

• Clonality: Polyclonal
• NCBI: 4659
• UniProt: O14974
• Purity: Affinity purification
• Sequence: RSTQG
• Target: PPP1R12A/PPP1R12B/PPP1R12C
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Signal Transduction,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Cell Cycle,Cytoskeleton,Motor Proteins,Actins.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Rabbit pAb (AP1164) at 1:1000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.