Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb

Product Overview

• Article Name: Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb
• Biozol Catalog Number: ABB-AP1171
• Supplier Catalog Number: AP1171
• Alternative Catalog Number: ABB-AP1171-100UL, ABB-AP1171-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: AMPKa1/AMPKa2, Phospho-AMPKa1/AMPKa2-T183/T172

Product Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Properties

• Molecular Weight: 64kDa/65kDa/62kDa
• NCBI: 5562
• UniProt: Q13131
• Purity: Affinity purification
• Target: PRKAA1/PRKAA2

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Protein phosphorylation.

Images

Western blot analysis of various lysates using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171) at 1:1000 dilution. 293T cells were treated with oligomycin (0. 5 uM) at 37°C for 30 minutes. C2C12 cells were treated with oligomycin (0. 5 uM) at 37°C for 30 minutes. C6 cells were treated with oligomycin (0. 5 uM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.