Phospho-BRD4-S498/S499 Rabbit pAb

Product Overview

• Article Name: Phospho-BRD4-S498/S499 Rabbit pAb
• Biozol Catalog Number: ABB-AP1179
• Supplier Catalog Number: AP1179
• Alternative Catalog Number: ABB-AP1179-20UL, ABB-AP1179-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: CAP, MCAP, HUNK1, HUNKI, FSHRG4, Phospho-BRD4-S498/S499

Product Description

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Product Properties

• Molecular Weight: 152kDa
• NCBI: 23476
• UniProt: O60885
• Purity: Affinity purification
• Target: BRD4

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Cancer,Tumor suppressors,Signal Transduction,Kinase,Serine threonine kinases.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-BRD4-S498/S499 Rabbit pAb (AP1179) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.