Phospho-HSF1-S326 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-HSF1-S326 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1189
• Supplier Catalog Number: AP1189
• Alternative Catalog Number: ABB-AP1189-20UL, ABB-AP1189-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HSTF1, Phospho-HSF1-S326

Product Description

The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.

Product Properties

• Molecular Weight: 57kDa
• NCBI: 3297
• UniProt: Q00613
• Purity: Affinity purification
• Target: HSF1

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Signal Transduction,MAPK-JNK Signaling Pathway,Cardiovascular,Heart,Cardiogenesis.

Images

Western blot analysis of lysates from MCF7 cells, using Phospho-HSF1-S326 Rabbit pAb (AP1189) at 1:500 dilution. MCF7 cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.