Phospho-LRP6-S1490 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-LRP6-S1490 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1191
• Supplier Catalog Number: AP1191
• Alternative Catalog Number: ABB-AP1191-20UL,ABB-AP1191-100UL,ABB-AP1191-500UL,ABB-AP1191-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ADCAD2, STHAG7, Phospho-LRP6-S1490

Product Description

This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 180kDa
• NCBI: 4040
• UniProt: O75581
• Purity: Affinity purification
• Sequence: PPSPA
• Target: LRP6
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,mTOR Signaling Pathway,Cell Biology Developmental Biology,Microtubules,Wnt -Catenin Signaling Pathway,ESC Pluripotency and Differentiation,Stem Cells.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-LRP6-S1490 Rabbit pAb (AP1191) at 1:500 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.