Phospho-NCF4/p40-phox-T154 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-NCF4/p40-phox-T154 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1193
• Supplier Catalog Number: AP1193
• Alternative Catalog Number: ABB-AP1193-20UL,ABB-AP1193-100UL,ABB-AP1193-1000UL,ABB-AP1193-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: NCF, CGD3, P40PHOX, SH3PXD4, Phospho-NCF4/p40-phox-T154

Product Description

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 39kDa
• NCBI: 4689
• UniProt: Q15080
• Purity: Affinity purification
• Sequence: PRTRK
• Target: NCF4
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Cancer,Signal Transduction,Cell Biology Developmental Biology,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-NCF4/p40-phox-T154 Rabbit pAb (AP1193) at 1:1000 dilution. Jurkat cells were treated with PMA/TPA (200 nM) at 37°C for 10 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.