Phospho-EGFR-Y1173 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-EGFR-Y1173 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1213
• Supplier Catalog Number: AP1213
• Alternative Catalog Number: ABB-AP1213-100UL,ABB-AP1213-20UL,ABB-AP1213-500UL,ABB-AP1213-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2, Phospho-EGFR-Y1173

Product Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 134kDa
• NCBI: 1956
• UniProt: P00533
• Purity: Affinity purification
• Target: EGFR
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Tumor biomarkers,Signal Transduction,G protein signaling,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,Cell Biology Developmental Biology,Growth factors,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway,Cardiovascular,Angiogenesis.

Images

Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1173 Rabbit pAb (AP1213) at 1:500 dilution. A-431 cells were treated with EGF (25 µg/mL) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.