Phospho-MAP3K5-S967 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-MAP3K5-S967 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1217
• Supplier Catalog Number: AP1217
• Alternative Catalog Number: ABB-AP1217-100UL,ABB-AP1217-20UL,ABB-AP1217-1000UL,ABB-AP1217-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ASK1, MEKK5, MAPKKK5, Phospho-MAP3K5-S967

Product Description

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 155kDa
• NCBI: 4217
• UniProt: Q99683
• Purity: Affinity purification
• Target: MAP3K5
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Signal Transduction,Kinase,Serine threonine kinases,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cytoskeleton,Actins,Death Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Cardiovascular,Heart,Hypertrophy.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-MAP3K5-S967 Rabbit pAb (AP1217) at 1:1000 dilution. Jurkat cells were treated with CIP(20uL/400ul) at 37°C for 1 hour. Jurkat cells were treated with Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.