Phospho-RIPK1/RIP-S166 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-RIPK1/RIP-S166 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1230
• Supplier Catalog Number: AP1230
• Alternative Catalog Number: ABB-AP1230-100UL,ABB-AP1230-20UL,ABB-AP1230-500UL,ABB-AP1230-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: RIP, RIP1, AIEFL, IMD57, RIP-1, Phospho-RIPK1/RIP-S166

Product Description

This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 76 kDa
• NCBI: 8737
• UniProt: Q13546
• Purity: Affinity purification
• Sequence: MWSKL
• Target: RIPK1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse, ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,Kinase,Serine threonine kinases,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology Inflammation,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway.

Images

Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1/RIP-S166 Rabbit pAb (AP1230) at 1:800 dilution. HT-29 cells were treated with Z-VAD (50uM), human TNF-alpha (50ng/ml) and SM-164 (200nM) at 37°C for 5 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.