Phospho-PDHA1-S293 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PDHA1-S293 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP1250
• Supplier Catalog Number: AP1250
• Alternative Catalog Number: ABB-AP1250-100UL,ABB-AP1250-20UL,ABB-AP1250-500UL,ABB-AP1250-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PDHA, PDHAD, PHE1A, E1alpha, PDHCE1A, Phospho-PDHA1-S293

Product Description

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 43kDa
• NCBI: 5160
• UniProt: P08559
• Purity: Affinity purification
• Sequence: GHSMS
• Target: PDHA1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Carbohydrate metabolism,Warburg Effect.

Images

Western blot analysis of various lysates using Phospho-PDHA1-S293 Rabbit pAb (AP1250) at 1:1000 dilution. 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.